Blood flow and blood oxygen are important physiological parameters of the organism， which reflect the functional state of the organism. For brain injury with rapid onset and pathological changes， taking cerebral blood flow and blood oxygen as parameters for monitoring head injury is conducive to the diagnosis， treatment and evaluation of head injury. The paper uses a combined measurement system constructed by spectroscopy analysis and laser speckle imaging technology to monitor the blood flow and blood oxygen in the process of lipopolysaccharide induced brain injury in mice， and further analyzes the consistency and difference of the treatment effects of brain injury with hypertonic saline and mannitol. Experimental results show that compared with the blood oxygen and blood flow value of the control group （1.09±0.075， 0.75±0.019）， lipopolysaccharide could increase the cerebral blood flow （1.36±0.080） and decrease the cerebral blood oxygen （0.62±0.021） in mice， which has significant difference. Blood oxygen and blood flow gradually recover after treatment， and the recovery is more obvious after hypertonic saline treatment. It is found that the blood flow and blood oxygen reveal an opposite trend after brain injury， which reflects the imbalance of blood brain maintenance caused by the change of the blood-brain barrier after brain injury. However， the blood flow and blood oxygen recover after the improvement of therapeutic agents， indicating that the blood flow and blood oxygen could be used as two important parameters for therapeutic evaluation. Therefore， the combined monitoring of blood oxygen and blood flow using spectroscopy analysis technology and laser speckle imaging technology provides a technical solution for real-time monitoring of brain injury and therapeutic evaluation.