A novel method for multi-target angle estimation and mutual coupling self-calibration of bistatic multiple-input multiple-output (MIMO) radar in spatial colored noise is presented. The receiving data are preprocessed by the fourth-order cumulants, which can suppress the spatial colored noise. The rotation invariant matrices of the transmit arrays and receive arrays are exploited by using the character of Toeplitz matrix and the ESPRIT method. Further, the transmit and receive angles can be estimated without pairing algorithm. Moreover, mutual coupling self-calibration is realized by using the estimated transmit and receive angles and the method of Lagrange multipliers. The effectiveness of the proposed method is verified with computer simulation.