Aiming at the dynamic interference problem in UAV data collection, this paper proposes a real-time optimization scheme for UAV flight trajectory. In the case of limited collection distance, by optimizing the UAV flight trajectory, the energy consumption of the UAV in the limited mission time is minimized. In order to avoid interference, the scheme is divided into two stages: initial trajectory planning and online trajectory optimization. In the initial trajectory planning stage, offline planning is carried out according to trajectory cost and corner energy consumption without considering interference; in the online trajectory optimization stage, on the basis of the initial trajectory, dynamic interference is considered, and an interference localization algorithm based on Markov prediction model is designed, and the interference potential field is also proposed to optimize the initial trajectory. Simulation analysis shows that the proposed scheme can effectively improve the anti-interference performance of UAV communication and improve the UAV data collection ability.